Japan's Unit 731 in Manchuria conducted biological and chemical warfare experiments on thousands of Chinese, Korean, and Allied prisoners — including vivisection without anaesthesia, deliberate infection with plague and cholera, and frostbite testing. An estimated 3,000 to 12,000 people were killed. In a controversial Cold War deal, the US granted immunity to Unit 731 researchers in exchange for their data, and Japan has never formally acknowledged the programme's full scope.

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